relictum and/or immunized with live vaccines containing different strains of Escherichia coli. Domestic canaries ( Serinus canaria domestica) were experimentally infected with P. In this study, we tested whether anti-microbiota vaccination in birds targeting Enterobacteriaceae within mosquito midguts modulates the mosquito microbiota and disrupt Plasmodium relictum development in its natural vector Culex quinquefasciatus. Anti-tick microbiota vaccines targeting commensal bacteria of the vector microbiota alter vector feeding and modulate the taxonomic and functional profiles of vector microbiome, but their impact on vector-borne pathogen development within the vector has not been tested. Vector microbiota may hold the key to vector-borne pathogen control, as mounting evidence suggests that the contributions of the vector microbiota to vector physiology and pathogen life cycle are so relevant that vectorial capacity cannot be understood without considering microbial communities within the vectors.

4School of Environmental Sciences, University of Guelph, Guelph, ON, CanadaĪnimal and human pathogens that are transmitted by arthropods are a global concern, particularly those vectored by mosquitoes (e.g., Plasmodium spp.

3SEPPIC Paris La Défense, La Garenne Colombes, France.

2ANSES, INRAE, Ecole Nationale Vétérinaire d’Alfort, UMR BIPAR, Laboratoire de Santé Animale, Maisons-Alfort, France.

1Nature Research Centre, Akademijos 2, Vilnius, Lithuania.

Justė Aželytė 1†, Alejandra Wu-Chuang 2†, Rita Žiegytė 1, Elena Platonova 1, Lourdes Mateos-Hernandez 2, Jennifer Maye 3, Dasiel Obregon 4, Vaidas Palinauskas 1* and Alejandro Cabezas-Cruz 2*

Birdgut wr